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Sequence data from GenBank were aligned with the newly obtained sequences using MAFFT version 7.308 (Katoh et al. 2002). Alignments of the rbcL and tufA sequences were manually adjusted using Se-Al v. 2.0a11 (Rambaut 2002) and gaps were removed manually. All sequences were submitted to the BOLD database (Barcode of Life Data Systems, v. 2.7; Ratnasingham and Hebert 2007) and aligned together with sequences obtained from GenBank (for GenBank accession numbers see Table 1). After alignment, sequences that did not fulfill the criteria of the barcode region (Barcode region is a 600 bp long fragment [18]) were removed. The final alignment consisted of 897 bp for the rbcL and 888 bp for the tufA. Alignments were deposited in TreeBASE (S3 Table).
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